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1,000 results • Page
1 of 20
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Views
Votes
Replies
0
votes
0
replies
11
views
Defined objects of type "S4" error in single cell analysis
Seurat
single-cell
DoubletFinder
single-nuclei
SeuratObject
51 minutes ago by
sarphilsar
• 0
0
votes
0
replies
16
views
Adjust pvalue in R with different approach give me all different outcome
statistics
pvalue
adjustment
R
1 hour ago by
Jonathan Yoou
▴ 60
0
votes
1
reply
23
views
Minimum RAM and Storage requirement for creating PSSM using ncbi-blast-2.2.30+-x64-linux
blast
ram
storage
pssm
updated 45 minutes ago by
GenoMax
141k • written 1 hour ago by
Nafi
• 0
0
votes
0
replies
12
views
What does 'bundle_uuid' refer to in this metadata sheet?
SRA
illumina
GEO
metadata
1 hour ago by
jeffrey.maurer.informatics
• 0
0
votes
0
replies
17
views
p-value in CIBERSORT
CIBERSORT
p-value
updated 1 hour ago by
Ram
43k • written 1 hour ago by
michelafrancesconi9
▴ 20
0
votes
0
replies
42
views
RNA editing sites based on DNA seq and RNA seq
RNA-editing
updated 1 hour ago by
Ram
43k • written 3 hours ago by
Ahiad Chen Zion
• 0
0
votes
0
replies
42
views
barcode of TCR-sequencing
barcode
4 hours ago by
yueli7
▴ 250
0
votes
0
replies
45
views
Somatic variant calling with Mutect2 using 2 matched-normals for one sample
cancer
mutect
variant-calling
GATK
somatic-variants
updated 3 hours ago by
Ram
43k • written 6 hours ago by
Alexandros
• 0
0
votes
1
reply
73
views
unable to get feature count results
featureCounts
updated 1 hour ago by
Ram
43k • written 6 hours ago by
Ravita
• 0
0
votes
0
replies
46
views
Subsetting and merging back Seurat Object brings different results
Seurat
6 hours ago by
Bine
▴ 60
0
votes
2
replies
75
views
KEGG Pathways
r
RNA-seq
30 minutes ago by
Sudip
• 0
0
votes
0
replies
48
views
Seurat object reductions (PCA, UMAP,..) after subsetting data
Seurat
7 hours ago by
Bine
▴ 60
0
votes
0
replies
49
views
Force PLINK2 to neglect chrX as the sex chromosome
PLINK2
7 hours ago by
8armed
• 0
1
vote
2
replies
109
views
Need help for downloading arabdopsis thaliana reference genome fasta file and gtf file
Arabidopsis-thaliana
gtf
reference-genome
updated 1 hour ago by
Ram
43k • written 9 hours ago by
Ravita
• 0
0
votes
0
replies
43
views
Gene Specific coverage from WGS data
linux
WGS
Bioinformatics
SARS
CoV2
10 hours ago by
Adyasha
• 0
0
votes
0
replies
48
views
human gut metagenome taxonomy profile table and KO profile table from big cohort
metagenome
gut
human
13 hours ago by
fanglujing
▴ 60
0
votes
0
replies
63
views
Are some metagenomes too complex for MAGs?
metagenomics
metagenome
MAGs
assembly
15 hours ago by
Jacob
• 0
0
votes
1
reply
112
views
Differential Accessibility
DiffBind
updated 19 hours ago by
Ram
43k • written 20 hours ago by
Shloka
• 0
0
votes
3
replies
198
views
Landmark gene selection in L1000.
L1000
landmark-gene
cmap
updated 5 hours ago by
GenoMax
141k • written 21 hours ago by
kim
• 0
3
votes
7
replies
229
views
FastQC Quality per tile and per sequence behaving strange after using Cutadapt
fastqc
cutadapt
illumina
tile
paired-end
23 hours ago by
salias
• 0
0
votes
0
replies
69
views
Using limma in methylation EM-seq?
EM-seq
methylation
1 day ago by
Lluís R.
★ 1.2k
0
votes
0
replies
79
views
Synteny analysis
bacterial
synteny
genome
updated 1 day ago by
GenoMax
141k • written 1 day ago by
Namil
• 0
0
votes
4
replies
179
views
Genbank File Format
gbkformat
8 hours ago by
alenew.am
• 0
0
votes
0
replies
161
views
Assist me in determining whether the analysis process using the limma package has been executed correctly
limma
ArrayExpress
DifferentialExpression
GEOquery
1 day ago by
SSSJec
• 0
0
votes
3
replies
171
views
Software to separate reads from different individuals
software
development
nanopore
updated 22 hours ago by
GenoMax
141k • written 1 day ago by
njornet
▴ 20
0
votes
0
replies
69
views
Seeking Guidance on Identifying Mutations in DARs from ATAC Data in Cancer Genomes
Genomics
Bioinformatics
ATACseq
CancerResearch
1 day ago by
David
• 0
0
votes
2
replies
138
views
How to update R on ubuntu
installation
update
R
apt-get
1 day ago by
Bosberg
▴ 50
0
votes
0
replies
87
views
LEfSe
LEfSe
1 day ago by
benkosta
• 0
0
votes
0
replies
74
views
How should I handle read counts derived from SGSeq when I want to build DEXSeqDataSet object
DEXSeq
DEXSeqDataSet
SGSeq
1 day ago by
Sara
▴ 30
0
votes
2
replies
128
views
Longitudinal analysis of subpopulations: which approach is better?
differential-expression
DEG
model
1 day ago by
Lluís R.
★ 1.2k
0
votes
0
replies
87
views
Comparing peptide sequences with MS/MS peptide data using MaxQuant
Transcriptomics
Mass
Bioinformatics
spectrometry
Proteins
1 day ago by
atharvakarkare14
▴ 20
0
votes
2
replies
172
views
What should I consider as FASTA for dataset?
PDB
FASTA
20 hours ago by
Nafi
• 0
0
votes
0
replies
75
views
Differential accessibility using DiffBinf
diffbind
1 day ago by
Shloka
• 0
0
votes
0
replies
83
views
vg call vs vg surject
vg
variation
graphs
updated 1 day ago by
GenoMax
141k • written 1 day ago by
aliraza3119
• 0
0
votes
1
reply
113
views
Can I merge Hi-C fastq files from different lanes?
GenomeAssembly
BWA-MEM2
Hi-C
updated 1 day ago by
GenoMax
141k • written 1 day ago by
Winter
• 0
0
votes
1
reply
186
views
Finding batch and outlayers
Pca
updated 1 day ago by
christopher medway
▴ 450 • written 1 day ago by
Tigran
• 0
2
votes
2
replies
179
views
PDB related issue
rcsb
pdb
updated 1 day ago by
noodle
▴ 580 • written 1 day ago by
Nafi
• 0
0
votes
9
replies
2.5k
views
6 follow
Cannot process all the reads in a fast5 file?
metagenome
base-calling
fastq
nanopore
updated 1 day ago by
Ram
43k • written 8 months ago by
Gio
• 0
0
votes
1
reply
149
views
Downloading full alignments from Pfam
pfam
updated 2 days ago by
GenoMax
141k • written 2 days ago by
bef1
• 0
0
votes
0
replies
108
views
adjusting for confounders in LMER in R
confounders
LMER
R
updated 1 day ago by
dariober
14k • written 2 days ago by
rene.j.erhardt
▴ 20
1
vote
3
replies
264
views
How to assign cell types after integration in scRNA
scRNA-seq
updated 1 day ago by
ATpoint
82k • written 2 days ago by
Francesco
▴ 10
0
votes
0
replies
127
views
STAR total splices (in Log.final) vs collapsed splice junctions (in SJ.out.tab)
STAR
2 days ago by
tnminh89
▴ 10
0
votes
0
replies
130
views
Filter low express genes in microarray data
microarray
2 days ago by
Chris
▴ 260
0
votes
0
replies
538
views
Correlation between cell type prediction scores and individual gene expression in spatial transcriptomic datasets
single-cell
Spatial-Transcriptomics
3 days ago by
biocellbio
• 0
0
votes
0
replies
196
views
Phasing VCF Files and Analyzing Reads with Multiple Variants
haplotypes
vcf
phasing
4 days ago by
HarperReed
• 0
1
vote
0
replies
152
views
Simulation of label-free bottom-up proteomics expression dataset
label-free
bottom-up
lc-ms
proteomics
4 days ago by
KABILAN
▴ 70
0
votes
0
replies
169
views
Running Phylogenetic Analysis With NCBI Genome
population-genetics
phylogenetic
updated 3 days ago by
Ram
43k • written 4 days ago by
SineWave
• 0
0
votes
0
replies
164
views
RNA-seq: full length gene
RNA-seq
updated 4 days ago by
Ram
43k • written 4 days ago by
Nargis
• 0
0
votes
0
replies
150
views
Haplotype Phased Assembly Contigs to Chromosome Annotations
Assembly
phased
Haplotype
Annotation
4 days ago by
turcoa1
• 0
0
votes
0
replies
159
views
Designing single-stable RNA molecules
structure
RNA
4 days ago by
Edna
• 0
1,000 results • Page
1 of 20
Recent Votes
Programmatically retrieving positions of protein active site residues
Programmatically retrieving positions of protein active site residues
A: Can Biopython Properly Import Fasta Headers With Spaces In Them?
Programmatically retrieving positions of protein active site residues
Answer: Polishing genome assembly
Answer: Integrate transcriptomic data and proteomics data.
Answer: Integrate transcriptomic data and proteomics data.
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Jonathan Yoou
▴ 60
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▴ 260
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Lluís R.
★ 1.2k
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miles.anderson
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Recent Replies
Comment: Programmatically retrieving positions of protein active site residues
by
me
▴ 760
This question fits in nicely with the [SIB Swiss Institute of Bioinformatics course on Enzyme information in UniProt][1] [1]: https://w…
Answer: Programmatically retrieving positions of protein active site residues
by
me
▴ 760
This can be done via SPARQL at the UniProt.org sparql endpoint PREFIX rdfs: <http://www.w3.org/2000/01/rdf-schema#> PREFIX fald…
Comment: KEGG Pathways
by
Sudip
• 0
No it does not. Do you know of a way that can help me achieve what I want?
Comment: Minimum RAM and Storage requirement for creating PSSM using ncbi-blast-2.2.30+-x
by
GenoMax
141k
UniRef file is 43G compressed so probably will become ~60G when uncompressed. You would likely be looking at at least that much +10-20% GB …
Comment: RNAseq one control two conditions, shared and exclusive genes
by
matteo.levorato
• 0
thank you for your comment, but yes running DESeq2 is the starting point. dds <- DESeqDataSetFromMatrix(countData=RawCounts, …
Comment: Need help for downloading arabdopsis thaliana reference genome fasta file and gt
by
Ram
43k
Please stop pasting a sentence in the `tags` field. That field is for subject matter keywords. I've fixed your posts this time, please be m…
Comment: KEGG Pathways
by
Ram
43k
> is the below code correct Does it give you what you need?
Comment: from row count to tpm
by
Ram
43k
Hi https://www.biostars.org/u/119503/ Why did you delete this post?
Comment: ERROR When Using Ensembl Plants Biomart Wget API
by
GenoMax
141k
A couple of hours back using `plants.ensembl.org` was generating a 504 error but it seems to be working now. wget -O result.txt 'http…
Comment: why renaming Idents in Seurat object doesn't work?
by
Assa Yeroslaviz
★ 1.8k
Don't understand this comment. I don't have any columns with this name.
Answer: ERROR When Using Ensembl Plants Biomart Wget API
by
Ben_Ensembl
★ 2.4k
Hi Dora, For plant species such as *A. thalina*, you should use the http://plants.ensembl.org/biomart service, so the start of your wget…
Answer: Introduce SNPs on FASTA
by
christopher medway
▴ 450
You could take a look at the mutfa command in [seqtk][1] [1]: https://docs.csc.fi/apps/seqtk/#usage
Answer: Introduce SNPs on FASTA
by
Michael
54k
If you have a VCF file with all the ALT alleles that you need to insert and a reference genome in FASTA, you can use [bcftools consensus][1…
Comment: Introduce SNPs on FASTA
by
Riccardo
▴ 10
Thank you very much. Is there a script or program that does it automatically?
Comment: Post-imputation plot
by
kl
▴ 10
This does not warrant redoing the imputation, though? Do you have any advice of what to take care of, in this circumstance? Thanks!
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