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Minimum RAM and Storage requirement for creating PSSM using ncbi-blast-2.2.30+-x64-linux

I am current trying to create pssm from FASTA using ncbi-blast-2.2.30+-x64-linux. I will use UniProt and UnirRef90 db for the purpose. What will be the minimum requirements for RAM and Storage for generating the PSSM? The information will help me a lot.

blast pssm

UniRef file is 43G compressed so probably will become ~60G when uncompressed. You would likely be looking at at least that much +10-20% GB of RAM.

Thank you for the reply

I have 16 gb of RAM then I can give a try then.

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