Minimum RAM and Storage requirement for creating PSSM using ncbi-blast-2.2.30+-x64-linux
I am current trying to create pssm from FASTA using ncbi-blast-2.2.30+-x64-linux. I will use UniProt and UnirRef90 db for the purpose.
What will be the minimum requirements for RAM and Storage for generating the PSSM? The information will help me a lot.
How can I speed up the download here? https://ftp.uniprot.org/pub/databases/uniprot/uniref/uniref90/uniref90.fasta.gz I'm running wget on our compute servers, on the head node, and my personal computer which …
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Hi, I'd like to remove all repeat-derived (like transposon proteins) from a UniProtKB/Swiss-Prot file (for instance ftp://ftp.uniprot.org/pub/databases/uniprot/current_release/knowledgebase/complete/uniprot_sprot.fasta.gz). Is there an easy way to do it? …
<p>I'm finding via a UniProt to Ensembl mapping (available, among other places, through <code>UniProtKB: <ftp://ftp.uniprot.org/pub/databases/uniprot/current_release/knowledgebase/idmapping/README</code>)> that certain IDs do not have Ensembl gene IDs in …
UniRef file is 43G compressed so probably will become ~60G when uncompressed. You would likely be looking at at least that much +10-20% GB of RAM.
Thank you for the reply
I have 16 gb of RAM then I can give a try then.