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1,000 results • Page
3 of 20
Sort: replies
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Votes
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20
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22
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13k
views
11 follow
Forum:
Nextseq for analysts
basespace
casava
bcl2fastq
nextseq
updated 14 months ago by
Ram
43k • written 9.9 years ago by
Madelaine Gogol
5.3k
27
votes
22
replies
5.7k
views
8 follow
Forum:
Recommendations of Linux distros for beginners in bioinformatics
training
linux
updated 12 months ago by
Ram
43k • written 6.5 years ago by
ropolocan
▴ 810
20
votes
21
replies
2.9k
views
6 follow
Forum:
Next Gen seq for Job interview
next-gen
updated 14 months ago by
Ram
43k • written 9.3 years ago by
gauravsingh.singh86
▴ 20
99
votes
21
replies
9.0k
views
14 follow
Forum:
How Has Bioinformatics Improved Over Time?
bioinformatics
updated 15 months ago by
Ram
43k • written 11.3 years ago by
Kate
▴ 370
42
votes
21
replies
15k
views
10 follow
Forum:
A Database Of Signatures Of Selection In The 1000 Genomes Dataset
1000genomes
human
selection
updated 14 months ago by
Ram
43k • written 10.2 years ago by
Giovanni M Dall'Olio
28k
29
votes
21
replies
6.3k
views
Forum:
PhD vs Field application scientist in industry
career
updated 13 months ago by
Ram
43k • written 7.8 years ago by
Govi988
▴ 80
35
votes
21
replies
3.1k
views
11 follow
Forum:
Should you restrict use of your software based on your political views?
software
updated 20 months ago by
Ram
43k • written 8.6 years ago by
Jean-Karim Heriche
27k
67
votes
21
replies
6.1k
views
8 follow
Forum:
Meta: Markdown in Biostars (now enabled)
meta
14 months ago by
Ram
43k
17
votes
21
replies
3.5k
views
8 follow
Forum:
bioinformatics thesis
thesis
updated 3.1 years ago by
Joe
21k • written 3.1 years ago by
eridanus
▴ 40
81
votes
21
replies
5.1k
views
16 follow
Forum:
[UPDATE] Thrown into a Bioinformatics Position
bioinformatics
updated 14 months ago by
Ram
43k • written 7.9 years ago by
Sinji
★ 3.2k
39
votes
21
replies
14k
views
10 follow
Forum:
Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 11 months ago by
Ram
43k • written 5.6 years ago by
Juke34
8.6k
66
votes
21
replies
6.9k
views
18 follow
Forum:
Is there anyone who was a pure biologist?
next-gen
updated 14 months ago by
Ram
43k • written 10.1 years ago by
Chong Tang
▴ 100
27
votes
21
replies
9.3k
views
9 follow
Forum:
Better for my career: Degree in computer science or Phd in bioinformatics?
computer-science
phd
updated 13 months ago by
Ram
43k • written 7.7 years ago by
BioinfGuru
★ 1.7k
36
votes
21
replies
19k
views
9 follow
Forum:
What Is Your Favorite Conserved Protein?
protein
conservation
updated 14 months ago by
Ram
43k • written 10.7 years ago by
Mary
11k
49
votes
20
replies
2.1k
views
10 follow
Forum:
First experiences matter
first-time
tolerance
updated 4 months ago by
Michael Love
★ 2.6k • written 4 months ago by
Mensur Dlakic
★ 27k
91
votes
20
replies
20k
views
15 follow
Forum:
Publications for individualized medicine in cancer by whole genome, exome or transcriptome sequencing
N-of-one
WGS
RNA-Seq
next-gen
Exome
updated 19 months ago by
Ram
43k • written 10.0 years ago by
Obi Griffith
20k
50
votes
20
replies
6.1k
views
18 follow
Forum:
Explain bioinformatics to a 10 year old
bioinformatics
updated 14 months ago by
Ram
43k • written 8.2 years ago by
novice
★ 1.1k
42
votes
20
replies
3.4k
views
11 follow
Forum:
What are the most popular NGS tools?
ensembl
NGS
updated 13 months ago by
Ram
43k • written 8.1 years ago by
Emily
23k
12
votes
20
replies
3.3k
views
Forum:
Starting a career in Bioinformatics
career
updated 14 months ago by
Ram
43k • written 7.9 years ago by
rupashree.choudhury
▴ 10
7
votes
20
replies
2.1k
views
Forum:
NGS RNA-Seq Analysis Pipeline Tophat vs STAR
STAR
RNA-Seq
TopHat
updated 12 months ago by
Ram
43k • written 3.4 years ago by
mikefeixu
▴ 10
47
votes
19
replies
14k
views
10 follow
Forum:
Is Software Development a key part of Bioinformatics?
Software-Development
updated 21 months ago by
Ram
43k • written 9.4 years ago by
xinuke
▴ 70
9
votes
19
replies
8.6k
views
Forum:
Careful!! Velvet Generates Different Assemblies From The Same Input Data And Same Input Parameters With Openmp Enabled.
velvet
genome
updated 14 months ago by
Ram
43k • written 10.5 years ago by
Rahul Sharma
▴ 660
57
votes
19
replies
28k
views
6 follow
Forum:
Attention: Bowtie2 And Multiple Hits
next-gen
bowtie
mapping
updated 15 months ago by
Ram
43k • written 11.6 years ago by
David Langenberger
11k
38
votes
19
replies
10k
views
10 follow
Forum:
Kallisto New RNA-seq quantification method discussion
RNA-Seq
updated 13 months ago by
Ram
43k • written 8.1 years ago by
morovatunc
▴ 550
45
votes
19
replies
2.9k
views
9 follow
Forum:
Where and how NGS techniques are heading for the next 5 years?
next-gen
NGS
updated 12 months ago by
Ram
43k • written 6.9 years ago by
lakhujanivijay
5.8k
49
votes
19
replies
7.3k
views
13 follow
Forum:
Why Are There No Proteomics Questions In Biostar?
proteomics
biostars
updated 15 months ago by
Ram
43k • written 13.5 years ago by
Jdnavarro
▴ 410
50
votes
19
replies
5.0k
views
7 follow
Forum:
Proposal: Biostar Wants To Run Your Ads. Feedback Requested.
biostars
updated 15 months ago by
Ram
43k • written 11.2 years ago by
Istvan Albert
100k
27
votes
19
replies
4.6k
views
10 follow
Forum:
Google genomics cloud platform ?
genome
cloud
updated 14 months ago by
Ram
43k • written 8.9 years ago by
GouthamAtla
12k
56
votes
19
replies
4.1k
views
9 follow
Forum:
How to apply for a job
resume
cover-letter
application
CV
updated 12 months ago by
Ram
43k • written 6.8 years ago by
Emily
23k
30
votes
19
replies
10k
views
6 follow
Forum:
Programming language use distribution from recent programs / articles
programming-language
programs
updated 12 months ago by
Ram
43k • written 7.0 years ago by
Eric Normandeau
11k
35
votes
19
replies
3.8k
views
6 follow
Forum:
For the general public seeking advice from this community
diagnosis
rare-disease
updated 20 months ago by
Ram
43k • written 8.6 years ago by
Mary
11k
35
votes
19
replies
4.0k
views
Forum:
Crowd Sourcing The 'Analysis' Of The 'Big Foot' Genome
bigfoot
genome
updated 15 months ago by
Ram
43k • written 11.3 years ago by
Will
4.5k
70
votes
19
replies
5.6k
views
13 follow
Forum:
Thrown Into A Bioinformatics Position
computational-analysis
updated 22 months ago by
Ram
43k • written 8.6 years ago by
System
▴ 170
34
votes
19
replies
7.9k
views
12 follow
Forum:
Programming Language In Bioinformatics
perl
python
updated 14 months ago by
Ram
43k • written 11.0 years ago by
AndreiR
▴ 260
38
votes
18
replies
4.3k
views
11 follow
Forum:
Should We Have A Biostar Conference Or Meeting?
conference
biostars
updated 15 months ago by
Ram
43k • written 10.9 years ago by
Michael
54k
36
votes
18
replies
8.2k
views
Forum:
How Does Genomebrowse Free Genome Browser From Golden Helix Compare?
visualization
genome-browser
updated 3.1 years ago by
natay
20 • written 11.1 years ago by
Victor Lyuboslavsky
▴ 140
21
votes
18
replies
1.8k
views
Forum:
Is it safe to keep our data in github
github
updated 11 months ago by
Ram
43k • written 4.0 years ago by
rprog008
▴ 70
29
votes
18
replies
3.3k
views
7 follow
Forum:
Should I quit PhD program?
education
career
research
phd
updated 12 months ago by
Ram
43k • written 6.0 years ago by
George
▴ 30
23
votes
18
replies
2.6k
views
9 follow
Forum:
How to imagine the future of bioinformatics?
bioinformatics
updated 13 months ago by
Ram
43k • written 7.7 years ago by
biotech
▴ 570
13
votes
18
replies
3.2k
views
6 follow
Forum:
Bioinformatics independent study group
Proteomic
RNA-Seq
Genomic
updated 12 months ago by
Ram
43k • written 6.7 years ago by
Leite
★ 1.3k
23
votes
18
replies
1.5k
views
Forum:
Versioning of Blast DB and Reproducible Research
database
blast
reproducible-research
updated 11 months ago by
Ram
43k • written 4.0 years ago by
makrez
▴ 60
72
votes
18
replies
7.5k
views
17 follow
Forum:
What Is One Book Every Bioinformatician Should Read?
books
updated 14 months ago by
Ram
43k • written 10.9 years ago by
Philipp Bayer
8.5k
52
votes
18
replies
6.4k
views
14 follow
Forum:
Do You Feel Good Or Bad About Putting Your Photo At Biostar?
meta
biostars
updated 15 months ago by
Ram
43k • written 10.7 years ago by
ugly.betty77
★ 1.1k
29
votes
18
replies
2.8k
views
12 follow
Forum:
what are the big questions in bioinformatics field nowadays
genome
updated 11 months ago by
Ram
43k • written 4.2 years ago by
t4192
▴ 20
9
votes
17
replies
3.0k
views
Forum:
“Systematic pattern of excess success"
paper
statistics
updated 14 months ago by
Ram
43k • written 9.5 years ago by
mikhail.shugay
3.5k
26
votes
17
replies
2.3k
views
9 follow
Forum:
GalaxyProject, why and why not?
galaxy
sequencing
pipeline
updated 13 months ago by
Ram
43k • written 8.2 years ago by
support
▴ 50
13
votes
17
replies
4.9k
views
8 follow
Forum:
A Universal/Common Biorep System
reputation
updated 14 months ago by
Ram
43k • written 10.5 years ago by
Obi Griffith
20k
17
votes
17
replies
2.0k
views
6 follow
Forum:
Journal for beginners
journal
updated 12 months ago by
Ram
43k • written 6.2 years ago by
heref
▴ 20
13
votes
17
replies
4.2k
views
6 follow
Forum:
Advice for undergraduate bioinformatics learners
advice
updated 21 months ago by
Ram
43k • written 8.4 years ago by
hanchen1996
▴ 10
29
votes
17
replies
2.3k
views
6 follow
Forum:
Can Biostars use question template like Github issue/PR?
meta
biostars
template
updated 13 months ago by
Ram
43k • written 7.2 years ago by
shenwei356
8.5k
1,000 results • Page
3 of 20
Recent Votes
A: How is the design in DESeq2 work?
Error in CIBERSORTx ($ operator is invalid for atomic vectors)
Answer: RNAseq coverage vs depth for transcript isoform expression?
constructing pangenome through psvcp
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
Answer: RNAseq coverage vs depth for transcript isoform expression?
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Recent Replies
Comment: Error in CIBERSORTx ($ operator is invalid for atomic vectors)
by
nguyenn6
• 0
did you ever figure it out? i'm having the same issue.
Comment: Traveler with Infernal mapping failed
by
anton.i.petrov
• 0
Hi Larissa! I am the lead developer of R2DT and I've just noticed this question. Could you please email help@r2dt.bio with your input seque…
Comment: constructing pangenome through psvcp
by
analyst
▴ 50
Fixed the issue by updating nucmer version.
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
Can you create a small test database (`makeblastdb`) from your query file and try to run the search against the same file and see if that w…
Comment: calculating genomic coverage/ base overlap in R
by
1769mkc
★ 1.2k
you can `dput(head(df)` for the both the data frame so that other can test for possible solutions
Comment: Broad and Narrow peaks
by
ATpoint
82k
What is your question? It's unclear.
Comment: Help with Biopython for Beginner
by
Joe
21k
One question before offering more solutions: are you trying to learn Python with this as an example problem, or are you trying to find the …
Answer: RNAseq coverage vs depth for transcript isoform expression?
by
Gordon Smyth
★ 7.2k
See the exploration of isoform estimation precision vs read length and sequencing depth in our paper: https://doi.org/10.1093/nar/gkad1167.…
Comment: How to handle duplicated genes in TCGA data?
by
txema.heredia
▴ 130
Why does the tool require gene symbols and not ensembl ids? Is it retrieving information from somewhere else? If so, your best bet is to fi…
Comment: how to treat the replicates while performing WGCNA
by
mavy
▴ 10
Thank you so much for your response . Really appreciate it. I will try the way you mentioned and will get back if I have any query
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
hophuquy0944
• 0
i have the same error, i used high performance computer, here is my code to set memory. srun --nodes=1 --ntasks=1 --cpus-per-task=24 -…
Comment: Overlapping Ranges within Granges object
by
Alex Reynolds
35k
Yes, `bedmap` will perform operations on one or two BED files.
Comment: B allele frequency (BAF)
by
aidangcruickshank
▴ 10
Apologies for resurrecting this post, but any idea what that functions "regions" paramater takes? Would it be a GRanges object? The documen…
Comment: How to handle duplicated genes in TCGA data?
by
Ngrin
• 0
Thanks @txema.heredia.The tool I am going to use only accepts gene symbols. This is the reason. So I cannot use ENS IDs.
Answer: Design for complex RNA-Seq experiment using Deseq2
by
swbarnes2
14k
This is a rather small experiment, I don't know that you can establish a meaningful baseline for each patient with a single pre sample, and…
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