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Help with treetime ancestral MissingDataError

Hi everyone.

I'm trying to use treetime ancestral option but I'm getting this error:

enter image description here

I've checked the alignment file (run_alignment_no_resis.001.fas) and the format looks correct.

Anyone know what could be the problem?

alignment treetime multifasta

I've checked and it looks that one of the sequences is longer than the rest. Could this be what is causing the error?

You have not really provided enough information to say definitively, but if your alignment has sequences of different lengths, then this is definitely something you have to fix before proceeding. All sequences in a multiple sequence alignment must be the same length (when including gaps), otherwise you don't actually have a proper alignment.

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