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.fas or .fasta to .axt for KaKs Calculator

How do I convert a .fas or .fasta file into .axt?

I tried to run the .fas/.fasta file of my aligned sequences that I edited thru BioEdit. But the Ka/Ks value results in N/A and now I'm stuck.

Example .fas file:enter image description here

axt kaks fasta
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updated by Ram Ram • written by JM • JM

I saw this thread but unfortunately I'm not familiar with Python(?).

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written by JM • JM

If you have an aligned fasta file as shown in the example in thread linked above you can use the commands shown in the thread. No programming needed. Replace your file name in place of example-file.fasta or file.fa depending on the answer you use.

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written by GenoMax GenoMax

It gave an error: "^ invalid char ''' in expression". I tried to fix it it but errors just keep on appearing.

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written by JM • JM

Can you show output of head -5 your_aligned.fa?

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written by GenoMax GenoMax

This is my .fas file: enter image description here

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written by JM • JM

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