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context/input is missing.
Hi Nora,
To get an answer you need to have a well formatted question, please follow these rules to make a clear question, How To Ask Good Questions On Technical And Scientific Forums
For now I will assume that you want to define intergenic regions from GTF/GFF file and maybe have the output in bed file.
if so follow this tutorial
https://davetang.org/muse/2013/01/18/defining-genomic-regions/