16 hours ago by
hannes.bongartz
•
0
2
votes
10
replies
625
views
0
votes
5
replies
183
views
2
votes
4
replies
190
views
1 hour ago by
Picasa
▴
640
1
vote
4
replies
237
views
0
votes
3
replies
168
views
0
votes
3
replies
196
views
updated 22 hours ago by
Pierre Lindenbaum
162k
•
written 1 day ago by
QX
•
0
1
vote
3
replies
160
views
updated 2 hours ago by
Bastien Hervé
5.3k
•
written 1 day ago by
Sara
▴
30
4
votes
2
replies
118
views
1
vote
2
replies
281
views
0
votes
1
reply
5
views
updated just now by
Pierre Lindenbaum
162k
•
written 2 minutes ago by
Hadia
•
0
0
votes
1
reply
328
views
0
votes
1
reply
2.9k
views
updated 23 hours ago by
Ram
44k
•
written 3.6 years ago by
andemexoax
•
0
0
votes
1
reply
188
views
0
votes
1
reply
103
views
0
votes
1
reply
69
views
updated 3 hours ago by
ATpoint
82k
•
written 6 hours ago by
carolofharvest
▴
40
2
votes
1
reply
115
views
updated 23 hours ago by
Jeremy Leipzig
22k
•
written 23 hours ago by
liaotsungjen
▴
10
0
votes
0
replies
79
views
0
votes
0
replies
68
views
22 hours ago by
niconps14
•
0
0
votes
0
replies
33
views
3 hours ago by
adi.gershon1
•
0
0
votes
0
replies
9
views
updated 10 minutes ago by
Pierre Lindenbaum
162k
•
written 33 minutes ago by
elbakri.fatimazahrae
•
0
0
votes
0
replies
72
views
0
votes
0
replies
63
views
0
votes
0
replies
71
views
20 hours ago by
marco.barr
▴
130
0
votes
0
replies
51
views
Recent Votes
Recent Locations •
All
Cambridge, MA, just now
Sweden, 1 minute ago
Italy, 3 minutes ago
Canada, 6 minutes ago
France/Nantes/Institut du Thorax - INSERM UMR1087, 9 minutes ago
Japan, 14 minutes ago
India, 22 minutes ago
Recent Awards •
All
Popular Question to
Pegasus
▴
100
Popular Question to
seta
★
1.9k
Popular Question to
melissachua90
▴
70
Popular Question to
Hamtaro
▴
50
Popular Question to
ericrkofman
▴
20
Scholar to
chrchang523
10k
Recent Replies
Comment: Harmony integration group.by.var parameter
by
Picasa
▴
640
For downstream analysis, after integration, my plan is to annotate each cluster/cell type and then perform a DGE analysis of KO versus WT f…
Comment: How to calculate cell type frequency between two groups in single cell data
by
Bastien Hervé
5.3k
That is correct
Answer: Setting a threshold on gene expression for subsetting (scRNA-seq)
by
ATpoint
82k
That depends on the data and the marker. Generally I would plot violins per cluster for the marker(s) and see whether one robustly overexpr…
Comment: Error when i use EVidenceModeler to do the genome annotation
by
peanut
•
0
thank you so much ,i get it
Comment: Number of non-ATCG nucleotides replaced by Salmon
by
Tonya S.
▴
10
Oops, yes, that must be where they are coming from. For some reason, I was thinking the genome was just soft-masked. How embarrassing! Than…
Answer: Number of non-ATCG nucleotides replaced by Salmon
by
Rob
6.6k
If there are no other signs that anything is awry, I probably wouldn't worry about this. Is it possible that these non-canonical nucleotid…
Comment: Introducing NovaDemux, a Sequence Demultiplexer that Increases Yield and Salvage
by
Brian Bushnell
20k
I examined nine 10B runs and five 25B runs (we just started getting 25B flowcells a few months ago). I did not pay close attention but it …
Comment: Introducing NovaDemux, a Sequence Demultiplexer that Increases Yield and Salvage
by
GenoMax
142k
> I don't know how widespread these issues are outside of JGI.
Very few small/medium sequencing centers likely have NovaSeq X. This is the…
Comment: Introducing NovaDemux, a Sequence Demultiplexer that Increases Yield and Salvage
by
Brian Bushnell
20k
Supporting figures, taken from some slides I prepared for internal use.
HDist 0, 1, and 2 correspond to 0, 1, and 2 mismatches allowed in …
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
hannes.bongartz
•
0
This works. Thank you so so much!
Comment: Harmony integration group.by.var parameter
by
jared.andrews07
★
17k
>I am not sure, but using "Sample_ID" might remove the differences between the conditions right ?
More than likely, it'd at least impact t…
Comment: Harmony integration group.by.var parameter
by
Picasa
▴
640
Thanks jared.andrews07 for your answer.
So, you are suggesting to use only "Donor" in the integration?
```r
RunHarmony(seu_obj, group.by.…
Comment: Single cell analysis: Unable to subset cells in seurat object using desired nFea
by
sc_analysis
•
0
I am not sure what should be the cut off. Looking at the vlnplot before subsetting i thought most of the cells are falling under 7500 nfeat…
Comment: How to calculate cell type frequency between two groups in single cell data
by
Sara
▴
30
Thank you for your comment and sorry if this question might be so basic.
How can I normalize the number of cells?
If I am not wrong the i…
Comment: Bowtie 1.3.1 alignment error as array 21720,23124 produces sam bam files
by
Deepthi
•
0
I unzipped the fastq files to check whether reads are trimmed adapter or not. I have checked the quality control using fastqc they are go…
Traffic: 1437 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6