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Bedtools [intersect][1] allows you to specify the fraction of overlap between two BED (or BAM) files using the F/f/r flags. You could split…
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Sorry, I should have been more specific. By "that list" I mean what's populated in the Therapeutic Agents tab when no filters are applied. …
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Not sure if you wanting to do like this-
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If you know what the boundaries of your construct look like then trim the left-end of the read using the tag on that end (`ktrim=l`). Then …
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> Not every read in your data is going to match a guide.
That is also true for sure. Let me clarify what I mean. For every read you should…
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I don't think every guide is supposed to show up in these experiments as far as I have seen. You will get some guides with 0 counts as you …
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