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How do you design primers with sequencing adapters to amplify CRISPR-edited regions?

Are there standard tools to use when one designs primers for CRISPR-based applications?

For example, when designing CRISPR guide RNAs how do people design amplicons? I have found Primer3Wrapper, but is there something more widely used?

primer-design crispr primer3
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updated by malikahsanali1998 • malikahsanali1998 • written by genomics0101 • genomics0101

hey, i am malik ahsan ali student of bioinformatics and its my 7 semester . kindly suggest me final year project topics .

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written by malikahsanali1998 • malikahsanali1998

Hey, please do not randomly add answers to threads. That could be considered spamming. We have a search function so you can find related threads, this has been asked many times before.

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written by ATpoint ATpoint

1 answer

There is CHOPCHOP from a group at CBU in Bergen. Is that what you are looking for?

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written by Michael Dondrup Michael Dondrup

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