How do you design primers with sequencing adapters to amplify CRISPR-edited regions?

Question

[genomics0101]

Are there standard tools to use when one designs primers for CRISPR-based applications?

For example, when designing CRISPR guide RNAs how do people design amplicons? I have found Primer3Wrapper, but is there something more widely used?

Answer 1

[Michael]

There is CHOPCHOP from a group at CBU in Bergen. Is that what you are looking for?