Dear all,

we have the last 4 seats available for the course "Introduction to CRISPR for ecology and evolution studies": https://www.physalia-courses.org/courses-workshops/course53/

Dates: online, October 9th-13th

This course aims to equip participants with the knowledge and skills necessary to effectively utilize the CRISPR-Cas9 system for evolutionary studies. . This course will include both fundamental theoretical lessons and hands-on sessions with practical examples of genomic data analysis. All data used in this course has been chosen from up-to-date scientific literature. It will focus on experiment design, selection of methods and data analysis. General “starting-point” bench protocols will be shared, and each day will include a “Discussion and Q&A” session where participants can fine-tune their own projects with the instructors.

LEARNING OUTCOMES

• Understand the fundamentals of the CRISPR-Cas9 genome-editing tool.

• Design a reliable CRISPR-based gene knockout experiment.

• Annotate a gene of interest in a non-model species.

• Design and evaluate species-specific single guide RNAs (sgRNAs).

• Efficiently synthesize sgRNA and assemble optimized Cas9/sgRNA mixes.

• Screen for G0 CRISPR mutants using a fast genotyping method.

• Design genotyping strategies according to project needs.

• Analyze Cas9-induced genomic variants by SANGER and Illumina deep sequencing.

For the full list of our courses and workshops, please have a look at: https://www.physalia-courses.org/courses-workshops/