Dear all,

Registrations are open for the 2nd edition of the online course "Introduction to CRISPR for Ecology and Evolution Studies".

Dates: 14-18 October

Course website: https://www.physalia-courses.org/courses-workshops/course53/

The course is intended for researchers interested in implementing the CRISPR-Cas9 tool in their own projects. There will be theoretical lectures and hands-on practical exercises using web-based tools, license-free software, and UNIX-based command line programs. Experience using command-line based programs will be advantageous, but not required. An introduction to UNIX commands will be provided.

At the end of this course, attendees will be in the position to:

• Understand the fundamentals of the CRISPR-Cas9 genome-editing tool.
• Design a reliable CRISPR-based gene knockout experiment.
• Functionally investigate a gene of interest in a non-model species.
• Design and evaluate species-specific guide RNAs (gRNAs).
• Efficiently synthesize gRNA and assemble optimized Cas9/gRNA mixes.
• Screen for CRISPR-derived mutants using a number of genotyping methods.
• Design genotyping strategies according to project needs.
• Analyze Cas9-induced genomic variants by Illumina deep sequencing.
• Critically interpret the results from a CRISPR-based KO experiment

For the full list of our courses and workshops, please visit: https://www.physalia-courses.org/courses-workshops/

Should you have any questions, please do not hesitate to contact us.