The amino acid motif QKRAA, when expressed on HLA-DRB1, carries
susceptibility to develop rheumatoid arthritis. This motif is the
basis of strong B and T cell epitopes. Furthermore, it is highly
overrepresented in protein databases, suggesting that it carries a
function of its own. To identify this function, we used QKRAA peptide
affinity columns to screen total protein extracts from Escherichia
coli. We found that DnaK, the E. coli 70-kD heat shock protein, binds
QKRAA. Of interest, DnaK has a natural ligand, DnaJ, that contains a
QKRAA motif. We found that QKRAA-containing peptides inhibit the
binding of DnaK to DnaJ. Furthermore, rabbit antibody to the QKRAA
motif can inhibit binding of DnaJ to DnaK. These data suggest that
QKRAA mediates the binding of E. coli chaperone DnaJ to its partner
chaperone DnaK.
Hi All, We know that UKBB is very useful, free-available, exciting and amazing genetic data for population genetics, epidemiology, and genetic evolution. I want to …
Hi All, I want to build a tree to reflect the relationship between multiple samples with the following style (Figure 1a, Nature Genetics, PMID: 27749841). …
Is this an assignment question?
How does one
blastpto such a short sequence and hope to get any useful results?QKRAA: https://www.ncbi.nlm.nih.gov/pubmed/9109425