How to merge pool-seq DNA samples
Hi colleagues, I am using 100 flies as a pool to extract DNA, but it turned out to be prone to contamination due to the large sample size. So I split the 100 flies into 4 tubes with 25 for each. In this way, I got a good results of DNA, all 260/280 were between 1.8-2.0 The problem now is how to merge the 4 tubes into 1 pool for sequencing while keeping equal amount of DNA contribution to the pool. The NanoDrop also reports the DNA concentration, can I merge the 4 tubes based on this value?( I heard DNA concentration from NanoDrop wasn't accurate) Thanks!
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wang.yiguan •
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Hello wang.yiguan!
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