mothods for analyzing exons area
i want to know is there any tool to seperate only functional area i.e exons from whole DNA sequence?
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So you want to extract exonic sequences from the reference fasta sequence?
s exactly !!!!
Go to biomart and you can download the gtf files containing the location of the exons. You can then easily extract it from the fasta files
You can download bed file for these exons using UCSC Table browser. The bed file will have chromosome name, start and end location for that exon. You can then use samtools faidx or Picard's ExtractSequences program to extract them.