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115,822 results • Page
1 of 2317
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0
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Long branch lengths, low bootstrap values and misalignments
mafft
muscle
iqtree
just now by
samyu.senthil
• 0
0
votes
3
replies
127
views
Haddock 2.4
Protein
Docking
10 minutes ago by
Preeti
• 0
0
votes
0
replies
11
views
adding new assemblies to previously built practical haplotype graph (v2)
pangenome
phg_v2
plants
PHG
31 minutes ago by
b.contreras.moreira
▴ 10
0
votes
2
replies
119
views
Beagle Phasing and Imputation
imputation
phasing
beagle
1 hour ago by
Duarte Molha
▴ 240
1
vote
0
replies
12
views
ENVO Ontology to Annotate Plain Text
natural
language
NL
ontology
envo
1 hour ago by
marie.harmel
▴ 10
0
votes
2
replies
106
views
Subsetting Seurat object based on list of cell barcodes
seurat
updated 1 hour ago by
fracarb8
★ 1.5k • written 11 hours ago by
bgbs
• 0
34
votes
16
replies
20k
views
14 follow
Tutorial:
TCGA UUIDS to TCGA barcode (SampleID) in R
next-gen
GDC
R
TCGA
updated 1 hour ago by
aUser
▴ 30 • written 7.5 years ago by
martinguerrerog89
▴ 310
0
votes
1
reply
30
views
Issues With Cram -> Fastq from GTEx v8 WGS
fastq
GTEx
cram
updated 1 hour ago by
Pierre Lindenbaum
160k • written 2 hours ago by
Vullnet
• 0
0
votes
2
replies
69
views
DEG (Differential expression gene) analysis
DEG
R
updated 2 hours ago by
dsull
★ 5.6k • written 2 hours ago by
sooni
▴ 20
0
votes
1
reply
94
views
Biobambam vs Picard in RNA Sequencing Analysis for BAM to FASTQ
RNA-seq
Biobambam
Picard
updated 3 hours ago by
Zhenyu Zhang
★ 1.1k • written 13 hours ago by
kcarey
• 0
791
votes
167
replies
138k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 8 weeks ago by
Biostar
2.5k • written 7.3 years ago by
Istvan Albert
100k
0
votes
0
replies
34
views
News:
Online course: Adaptation Genomics
StructuralVariants
Selection
AdaptationGenomics
PopulationStructure
3 hours ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
32
views
News:
Online course: Analysis of Prokaryotic Pangenomes
BacterialGenomics
Pangenomics
3 hours ago by
carlopecoraro2
★ 2.4k
0
votes
1
reply
63
views
library(scater)
sce
scRNASeq
scater
updated 4 hours ago by
Mensur Dlakic
★ 26k • written 5 hours ago by
Nitin
• 0
0
votes
1
reply
160
views
Is it better to pool samples prior to running deseq2, or should the algorithm be run separately for every pool?
deseq2
RNA-seq
updated 7 hours ago by
swbarnes2
14k • written 10 hours ago by
Shelby
• 0
0
votes
4
replies
221
views
Expert Guidance Required for De Novo Transcriptome Analysis
Transcriptome
De-novo
updated 7 hours ago by
Doomhammer
• 0 • written 22 hours ago by
ben@f
▴ 10
0
votes
1
reply
493
views
Downloading epigenetic and mutation data of specific TCGA BRCA samples
Mutation
RNA-Seq
TCGA
updated 8 hours ago by
aUser
▴ 30 • written 2.6 years ago by
Soura
• 0
4
votes
4
replies
205
views
Differential express genes in imbalance groups
Limma
9 hours ago by
Chris
▴ 260
2
votes
1
reply
104
views
basal gene expression level
RNA-Seq
updated 13 hours ago by
LChart
3.8k • written 13 hours ago by
Francois Piumi
▴ 40
0
votes
0
replies
62
views
Evaluating MAST model fit using AIC
cell
single
MAST
scRNA
transcriptomics
13 hours ago by
mhale
• 0
1
vote
1
reply
120
views
Seurat 5 integration
integration
seurat
updated 14 hours ago by
jared.andrews07
★ 16k • written 16 hours ago by
sarahmanderni
▴ 100
3
votes
5
replies
172
views
scRNAseq normalization
scRNA-seq
SCT
updated just now by
ATpoint
81k • written 15 hours ago by
sarahmanderni
▴ 100
3
votes
7
replies
1.2k
views
7 follow
Transcription Start Site
TSS
updated 6 hours ago by
Ming Tommy Tang
★ 3.8k • written 23 months ago by
Fatemeh Nabizadeh
▴ 10
0
votes
0
replies
68
views
Limma contrasts - difference between single and multiple concurrent contrasts results
R
limma
updated 15 hours ago by
Ram
43k • written 15 hours ago by
shasabhi1
• 0
0
votes
0
replies
70
views
Genotype simulation
gwas
simulation
sim1000
updated 17 hours ago by
Ram
43k • written 17 hours ago by
Eliza
▴ 30
0
votes
0
replies
60
views
Tabula Sapiens and scanpy normalised expression data
scanpy
scRNA-seq
10x
normalisation
updated 17 hours ago by
Ram
43k • written 17 hours ago by
matt.a.bennett25890
• 0
4
votes
2
replies
135
views
Problem with .bed file
cygwin64
bed
PLINK
updated 14 hours ago by
Ram
43k • written 18 hours ago by
Dinmukhamed
• 0
0
votes
1
reply
175
views
compareCluster's enrichGO difference in results when stating ont = "ALL" vs NULL
enrichGO
clusterProfiler
updated 18 hours ago by
Dunois
★ 2.5k • written 1 day ago by
TIANTHING JESTLIN
• 0
0
votes
0
replies
74
views
Remove SNPs based on MAF in Fst analysis
SNP
MAF
Fst
Popgenome
19 hours ago by
kopelol
▴ 20
4
votes
2
replies
232
views
Before or after LD clumping for PRS calculation in GWAS
GWAS
3 hours ago by
경준
▴ 20
1
vote
2
replies
238
views
Trim Galore: Automatic detection of adapters
trimgalore
13 hours ago by
Daniel
▴ 30
1
vote
1
reply
95
views
Troubleshooting a REGENIE sex-stratified GWAS
GWAS
REGENIE
updated 30 minutes ago by
Corentin
▴ 600 • written 20 hours ago by
s.may-wilson
▴ 50
0
votes
3
replies
192
views
Average expression of a sample in single-cell data
single-cell
rna-seq
updated 6 hours ago by
dsull
★ 5.6k • written 20 hours ago by
newuser2024
• 0
0
votes
4
replies
230
views
blastn: 2.2.31+ Package: blast 2.2.31, build Jun 2 2015 10:47:46
blastn
blast
ubuntu
updated 17 hours ago by
SequenceServer
▴ 140 • written 23 hours ago by
Luca
• 0
0
votes
2
replies
153
views
nf-core/rnaseq, problem with ensembl reference
nfcore
nextflow
ensembl
rnaseq
17 hours ago by
Filip
• 0
0
votes
1
reply
123
views
Deeptools scale issue for plotHeatmap
deeptools
computeMatrix
plotHeatmap
updated 17 hours ago by
Ram
43k • written 23 hours ago by
Learner
• 0
1
vote
1
reply
108
views
turning old shell ncbi querys into url querys
eutils
ncbi
updated 22 hours ago by
GenoMax
140k • written 23 hours ago by
Guelakais
• 0
0
votes
1
reply
453
views
ArchR Doublet Inference to Signac
Signac
scATAC-seq
ArchR
updated 1 day ago by
fracarb8
★ 1.5k • written 13 months ago by
bioinformatics.girl
▴ 20
0
votes
2
replies
156
views
calculating mean fragment length from CollectInsertSizeMetrics(Picard) output
count2fpkm
21 hours ago by
Niharika
• 0
3
votes
10
replies
626
views
t-test in two groups, multiple rows
R
t-test
9 hours ago by
sooni
▴ 20
3
votes
4
replies
1.3k
views
sam.gz files to bam using samtools
RNA-Seq
samtools
updated 1 day ago by
robertoalbanese.bioinfo
• 0 • written 12 months ago by
tikshyadav19
• 0
0
votes
4
replies
183
views
error in wigToBigWig while converting mappability to bedgraph
mappability
gem
bedgraph
wigToBigWig
1 hour ago by
Dora
▴ 10
0
votes
0
replies
100
views
Forum:
Field of Study in Computational Biology
Genomics
Computational-Biology
PhD
updated 17 hours ago by
Ram
43k • written 1 day ago by
Mohammed
• 0
0
votes
2
replies
166
views
FASTA/FASTQ files of fetal Aneuploids
nipt
22 hours ago by
lolbye_1
• 0
0
votes
0
replies
109
views
comparing sc-seq combination and bulk-sequencing
bulk-sequencing
sc-sequencing
1 day ago by
润达
• 0
1
vote
1
reply
197
views
Finding homologous protein sequence using blastp led to the same sequence as output
Blastp
updated 1 day ago by
Mensur Dlakic
★ 26k • written 1 day ago by
hamid sta
▴ 10
1
vote
1
reply
152
views
Where to download DOCK 6.11?
docking
updated 1 day ago by
ATpoint
81k • written 1 day ago by
tsakeliz
• 0
0
votes
1
reply
145
views
BRIG coverage.graph issues
BRIG
sam
updated 1 day ago by
Pierre Lindenbaum
160k • written 1 day ago by
dlera.lozano
• 0
0
votes
0
replies
116
views
How to generate a comparative map using CGview?
genome
map
CGView
1 day ago by
marongiu.luigi
▴ 710
0
votes
0
replies
130
views
Advice on telling if two SNPs are on the same haplotype
ldpair
halplotype
co-occurance
gnomad
snp
1 day ago by
amy__
▴ 160
115,822 results • Page
1 of 2317
Recent Votes
A: Normalize FPKM values for cross-sample comparisons?
Answer: ColabFold
C: How To Convert Arabidopsis Gene Id To Ncbi Geneid, Ncbi-Gi Or Uniprot?
Troubleshooting a REGENIE sex-stratified GWAS
ENVO Ontology to Annotate Plain Text
Answer: Seurat 5 integration
Comment: scRNAseq normalization
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Recent Replies
Comment: scRNAseq normalization
by
ATpoint
81k
That is an entirely different analysis and depends on the testing Framework. If you use the commonly-used Wilcox test or something like lim…
Answer: Haddock 2.4
by
Preeti
• 0
i have three different runs with haddock score of -75, -68, -61 . Which one is better among these( molecule 1 is same in all three ,only mo…
Comment: NGS/GATK pipeline returns too many variants
by
dhruti
• 0
@dodausp Did you ever find a reason for this? I am having the same problem
Comment: Troubleshooting a REGENIE sex-stratified GWAS
by
Corentin
▴ 600
Sex chromosomes are always a bit tricky to handle in associations studies. How many male cases and male controls you have for this phenoty…
Answer: Haddock 2.4
by
Preeti
• 0
I want to know that which results from haddock are good like I got highest haddock score of -75 .....is it a good haddock score or not
Comment: Beagle Phasing and Imputation
by
Duarte Molha
▴ 240
Hi @lchart Thank you for your answer. I coded my male sample as biallelelic homozygous as i was aware of limitations for allosomes.…
Comment: error in wigToBigWig while converting mappability to bedgraph
by
Dora
▴ 10
And I also change the chrom name in the .sizes file. That works perfect. Thank you very much!
Comment: scRNAseq normalization
by
sarahmanderni
▴ 100
Thanks for the response! I also was wondering to ask if you have found the log-norm also working fine in terms of downstream analysis like …
Answer: Subsetting Seurat object based on list of cell barcodes
by
fracarb8
★ 1.5k
`subset(seurat.obj_combined, cells = all_good_cells_rhemac10$V1)` You need to pass `cells` not `subset`. From the documentation: sub…
Answer: TCGA UUIDS to TCGA barcode (SampleID) in R
by
aUser
▴ 30
Great solutions on this page, the old web page has been replace with new one, so you need to use the new link, and it will work: old lin…
Comment: Issues With Cram -> Fastq from GTEx v8 WGS
by
Pierre Lindenbaum
160k
unless your cram in sorted on query rather than on coordinate, your cram must be first sort processed with collate: https://twitter.com/lh…
Answer: error in wigToBigWig while converting mappability to bedgraph
by
Pierre Lindenbaum
160k
remove the spaces from your "chrom" key variableStep chrom=NT_173277 span=5 or may be quote the chrom variableStep chrom="NT_173277.1 C…
Comment: DEG (Differential expression gene) analysis
by
dsull
★ 5.6k
Well, I guess expression could be measured from the DNA indirectly such as with RNA Pol II binding to DNA via ChIP-seq but then the OP shou…
Answer: DEG (Differential expression gene) analysis
by
dsull
★ 5.6k
What exactly is "DNA-level DEG"? That makes zero sense. DEG = Differential **EXPRESSION** of genes. In any case, for RNA sequencing data, …
Answer: Biobambam vs Picard in RNA Sequencing Analysis for BAM to FASTQ
by
Zhenyu Zhang
★ 1.1k
Let me say even if you had used the exactly same bioinformatics steps, you will still have batch efforts from sample collection, storage an…
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