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1,000 results • Page
2 of 20
Sort: replies
Rank
Views
Votes
Replies
16
votes
21
replies
2.3k
views
Why there are different hit for a same gene in different species
gene
blast
ortholog
paralog
7.6 years ago by
Farbod
★ 3.4k
6
votes
21
replies
2.8k
views
How to evaluate the similarity of genes between two sample
RNA-Seq
differential
updated 5.6 years ago by
Biostar
20 • written 5.7 years ago by
afli
▴ 190
5
votes
20
replies
4.2k
views
DiscoSnp Segmentation fault
snp
discosnp
updated 19 months ago by
Ram
43k • written 8.7 years ago by
Hans
▴ 140
6
votes
20
replies
6.4k
views
filling of missing genotype information in merged variant call vcf file
SNP
5.9 years ago by
princy149
▴ 80
8
votes
20
replies
3.0k
views
Why does cufflinks split this transcript?
RNA-Seq
cufflinks
assembly
6.3 years ago by
corend
▴ 70
8
votes
20
replies
2.7k
views
Getting read depth for normal and tumour
R
WGS
vcf
5.1 years ago by
zizigolu
★ 4.3k
2
votes
20
replies
2.4k
views
The number of SVs called by `vg call` is much smaller than the number of SVs in the VCF used to construct the graph
vg
7 months ago by
Maxine
▴ 40
0
votes
20
replies
1.6k
views
Rockhoppper - Suspiciously low percentage of perfectly aligned reads
Rockhopper
RNA-seq
6 months ago by
langziv
▴ 50
5
votes
20
replies
8.3k
views
How to plot ChIP-seq Density vs Distance from TSS using Homer annoted files
ChIP-Seq
homer
7.7 years ago by
varsha619
▴ 90
22
votes
20
replies
2.6k
views
what is the prefered strategy to treat with blast-less transcriptomes from RNA-seq
blast
RNA-Seq
genome
gene
updated 12 months ago by
Ram
43k • written 7.8 years ago by
Farbod
★ 3.4k
9
votes
20
replies
4.3k
views
Solve SAM issues flagged by Picard's ValidateSamFile
Picard
SAM
updated 13 months ago by
Ram
43k • written 5.7 years ago by
marongiu.luigi
▴ 710
8
votes
20
replies
2.5k
views
Converting runpsipred Script to Work on Windows OS
psipred
windows
updated 23 months ago by
Ram
43k • written 5.8 years ago by
Bara'a
▴ 270
4
votes
20
replies
1.9k
views
How can I count aminoacid residues from a HUGE compressed fasta file?
sequence
4.3 years ago by
schlogl
▴ 160
6
votes
19
replies
2.9k
views
Clustering for Single-cell RNA-seq Data
clustering
R
single-cell
4.8 years ago by
aloke205
▴ 40
3
votes
19
replies
1.5k
views
Systematic errors at the end and beginning of reads in NGS panels
NGS
error
panels
sequencing
updated 10 months ago by
Asaf
10k • written 10 months ago by
captainlabman
▴ 20
9
votes
19
replies
2.3k
views
There are any way to use a script for feed emboss with fasta sequence?
sequence
4.3 years ago by
schlogl
▴ 160
6
votes
19
replies
1.7k
views
Fastq header modification
sequence
next-gen
updated 6.1 years ago by
Ram
43k • written 6.1 years ago by
Guillaume
• 0
0
votes
19
replies
1.5k
views
best blast strategy: read vs cluster?
alignment
blast
search
strategy
4.4 years ago by
marongiu.luigi
▴ 710
0
votes
19
replies
3.8k
views
Difficulty installing GATKtoolkit
GATK
Variant calling
SNPs
Indels
6.3 years ago by
jaqx008
▴ 110
1
vote
19
replies
2.1k
views
High no feature counts in ht-seq counts
RNA-Seq
sequencing
5.4 years ago by
eozcan
▴ 10
0
votes
19
replies
1.4k
views
map fasts files
RNA-Seq
FASTQ
R
6.2 years ago by
Learner
▴ 280
0
votes
19
replies
2.0k
views
scatterplot in R
microarray
expression
gene
updated 19 months ago by
Ram
43k • written 19 months ago by
bioinformatics
▴ 40
5
votes
19
replies
1.7k
views
how I can come up with a permanent error
R
software error
7.0 years ago by
zizigolu
★ 4.3k
10
votes
19
replies
3.5k
views
What is a difference between uniprot and 'nr. Non-redundant GenBank...'?
Genebank
blast
UniProt
updated 3.0 years ago by
GenoMax
141k • written 3.0 years ago by
matt
▴ 20
18
votes
19
replies
2.3k
views
6 follow
Extremely important marker gene has many reads based on IGV viewer but no quantified reads
rna-seq
sequencing
igv
alignment
next-gen
updated 17 months ago by
Ram
43k • written 5.3 years ago by
rbkh09
• 0
3
votes
19
replies
6.1k
views
gatk-launch file missing
next-gen
sequencing
software error
updated 5.0 years ago by
finswimmer
16k • written 5.0 years ago by
sruthi
▴ 40
4
votes
19
replies
3.1k
views
Use machine learning as classifier
R
rRNA
16S
18S
ITS
updated 6.7 years ago by
Biostar
20 • written 6.8 years ago by
cool.abbecker
▴ 30
17
votes
19
replies
2.8k
views
Sorting sequences according header
software error
sequence
Assembly
next-gen
7.7 years ago by
Gian77
▴ 60
1
vote
19
replies
885
views
Matching transcriptomic data to clinical data
R
updated 12 weeks ago by
Ram
43k • written 12 weeks ago by
Khadija
▴ 10
7
votes
19
replies
2.9k
views
mapping script process sleeping on server.
alignment
updated 23 months ago by
Ram
43k • written 9.2 years ago by
lvogel
▴ 30
0
votes
19
replies
2.8k
views
error related to vcfstats
numpy
snp
vcf
vcfstats
2.7 years ago by
rheab1230
▴ 140
0
votes
19
replies
9.6k
views
Skip orientation as there are not enough pairs by bwa mem
whole genome
bwa
paired end sequencing
4.9 years ago by
seta
★ 1.9k
22
votes
19
replies
3.2k
views
targets of a list of miRNAs
miRNAs
RNA-Seq
DEanalysis
target-prediction
7.6 years ago by
zizigolu
★ 4.3k
7
votes
19
replies
7.0k
views
[R] Microarray analysis interpreting logFC after makeContrasts
microarray
R
updated 2.3 years ago by
Ram
43k • written 9.5 years ago by
mheiser1
▴ 10
5
votes
19
replies
4.0k
views
8 follow
Hisat2 aligner problem
RNA-Seq
ChIP-Seq
alignment
Assembly
software error
updated 4 months ago by
ATpoint
82k • written 4.9 years ago by
ta_awwad
▴ 340
1
vote
19
replies
7.1k
views
Conda install package - different internals
R
software error
updated 4.7 years ago by
Biostar
20 • written 4.7 years ago by
lihe.liu
▴ 30
13
votes
19
replies
5.4k
views
bbmerge not joining paired-end reads
bbmerge
alignment
6.4 years ago by
bioplanet
▴ 60
15
votes
19
replies
1.9k
views
trimmomaric command for a fasta file?
trimmomatic
updated 6.0 years ago by
Ram
43k • written 6.0 years ago by
Nadin.asal
• 0
2
votes
18
replies
6.2k
views
No differentially expressed genes using DESeq2
RNA-Seq
deseq2
7.5 years ago by
Sumit Paliwal
▴ 40
2
votes
18
replies
2.4k
views
I need help in this
gene
weblems
alignment
updated 21 months ago by
Ram
43k • written 8.3 years ago by
efosa15
• 0
4
votes
18
replies
17k
views
Alignment with BWA and SAMtools
BWA
SAM-file
SAMtools
updated 3.5 years ago by
Biostar
20 • written 5.7 years ago by
Shelle
▴ 30
0
votes
18
replies
1.9k
views
freebayes error variant calling
snp
3.7 years ago by
evelyn
▴ 230
7
votes
18
replies
4.9k
views
Fold change UP and Down in dplyr calculation
R
6.7 years ago by
1769mkc
★ 1.2k
14
votes
18
replies
2.2k
views
Clustering in single cell
seurat
single-cell
updated 6 months ago by
e.r.zakiev
▴ 200 • written 7 months ago by
Chris
▴ 260
1
vote
18
replies
2.7k
views
Why big gaps when I use Entrez Eutils to download protein coding sequences.
entrez
eutils
7.7 years ago by
Tom
▴ 40
0
votes
18
replies
1.6k
views
No gene name after annovar vcf file processing
vcf
annovar
4.5 years ago by
valerie
▴ 100
7
votes
18
replies
4.6k
views
Bowtie indexing of a fasta file that consists of a large amount of sequences
bowtie
alignment
genome
7.5 years ago by
valerie
▴ 100
4
votes
18
replies
3.0k
views
Inline barcodes in the reverse reads
barcodes
7.5 years ago by
Picasa
▴ 640
5
votes
18
replies
6.8k
views
EnhancedVolcano plot error: Log2foldchange is not numeric!
R
EnhancedVolcano
4.4 years ago by
michelle.piquet
▴ 60
4
votes
18
replies
4.1k
views
Significance of reads mapping to Viruses - FusionCatcher
RNA-Seq
virus
reads
fusion
updated 21 months ago by
Ram
43k • written 8.4 years ago by
Joel TM
▴ 60
1,000 results • Page
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Recent Votes
CTO/founding engineer role at Voyant Bio
Programmatically retrieving positions of protein active site residues
Programmatically retrieving positions of protein active site residues
A: Can Biopython Properly Import Fasta Headers With Spaces In Them?
Programmatically retrieving positions of protein active site residues
Answer: Polishing genome assembly
Answer: Integrate transcriptomic data and proteomics data.
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Recent Replies
Comment: KEGG Pathways
by
Ram
43k
Give us as much detail as you can. What error does it give you?
Comment: What does 'bundle_uuid' refer to in this metadata sheet?
by
GenoMax
141k
SRA Run Selector shows a better view of the metadata: https://www.ncbi.nlm.nih.gov/Traces/study/?acc=PRJNA761679&o=acc_s%3Aa
Comment: Adjust pvalue in R with different approach give me all different outcome
by
Aspire
▴ 300
- p.adjust() is supposed to receive a **numeric vector of p-values**, not a matrix. - It makes no sense to "adjust" just one p-value, as…
Comment: Downsampling fastq file
by
marco.barr
▴ 80
I understood where the problem lies. I discussed with my wet lab colleagues (this is part of a bioinformatician's job...) and by showing th…
Comment: Programmatically retrieving positions of protein active site residues
by
me
▴ 760
This question fits in nicely with the [SIB Swiss Institute of Bioinformatics course on Enzyme information in UniProt][1] [1]: https://w…
Answer: Programmatically retrieving positions of protein active site residues
by
me
▴ 760
This can be done via SPARQL at the UniProt.org sparql endpoint PREFIX rdfs: <http://www.w3.org/2000/01/rdf-schema#> PREFIX fald…
Comment: KEGG Pathways
by
Sudip
• 0
No it does not. Do you know of a way that can help me achieve what I want?
Comment: Minimum RAM and Storage requirement for creating PSSM using ncbi-blast-2.2.30+-x
by
GenoMax
141k
UniRef file is 43G compressed so probably will become ~60G when uncompressed. You would likely be looking at at least that much +10-20% GB …
Comment: RNAseq one control two conditions, shared and exclusive genes
by
matteo.levorato
• 0
thank you for your comment, but yes running DESeq2 is the starting point. dds <- DESeqDataSetFromMatrix(countData=RawCounts, …
Comment: Need help for downloading arabdopsis thaliana reference genome fasta file and gt
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Please stop pasting a sentence in the `tags` field. That field is for subject matter keywords. I've fixed your posts this time, please be m…
Comment: KEGG Pathways
by
Ram
43k
> is the below code correct Does it give you what you need?
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43k
Hi https://www.biostars.org/u/119503/ Why did you delete this post?
Comment: ERROR When Using Ensembl Plants Biomart Wget API
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141k
A couple of hours back using `plants.ensembl.org` was generating a 504 error but it seems to be working now. wget -O result.txt 'http…
Comment: why renaming Idents in Seurat object doesn't work?
by
Assa Yeroslaviz
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Don't understand this comment. I don't have any columns with this name.
Answer: ERROR When Using Ensembl Plants Biomart Wget API
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Hi Dora, For plant species such as *A. thalina*, you should use the http://plants.ensembl.org/biomart service, so the start of your wget…
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