How To Calculate Read Count For Antisense Study?
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Do you need to perform the read counts in R? If not, you can easily count the number of reads in a FASTQ file by typing, in bash
wc -l [your unzipped FASTQ file]and dividing the result by four. If the file is gzipped, then just typezcat [your gzipped FASTQ file] | wc -lHi Deedee,
I know how to count read in fastq file. My question is how to calculate them to identifying antisense from mapped data