What preliminary steps should I do before checking the quality of sequence for RNA-seq
I have RNAseq data for human and mouse, and I am going to start RNA-seq analysis, my data is single end with 100nt. Is there any preliminary steps should I do before checking the quality of sequence, trim adapter ,and, map the reads to human and mouse genome.
2 answers
There's nothing that needs to be done prior to QC/trimming.
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Ram
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Devon Ryan
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Devon Ryan
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