Best approach for miRNA-seq when species is not in miRBase
Hi all,
I am working with small RNA-seq data from Mesocricetus auratus (Syrian hamster) and noticed that it is not available as a standard species in miRBase.
What would be the best way to proceed with miRNA analysis in this case? Would you recommend:
If anyone has experience working with hamster miRNA data or a similar case, I would really appreciate your suggestions on the most reliable approach.
Thank you in advance for your help!
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While syrian hamster may not be in the miRBase, it looks like there is a recent paper that appears to have identified 20K+ miRNA. See --> https://pmc.ncbi.nlm.nih.gov/articles/PMC11931762/
Do you have your own miRNA data or are you looking to get practical guidance on how to do miRNA sequencing.