I tried to download some fasta files from ENA today with following code:

cat fq.txt |while read id;
do ascp -QT -l 300m -P33001 -k 1 -v -i /home/tomas/.aspera/connect/etc/asperaweb_id_dsa.openssh era-fasp@${id} . ;
done

One example line in my fq.txt:

fasp.sra.ebi.ac.uk:/vol1/fastq/ERR249/001/ERR2497991/ERR2497991_1.fastq.gz

But it comes with ascp: failed to authenticate.

I'm sure the openssh exists in the path.

My code works well one or two weeks ago. I don't know why this happens. Does anyone know what's the problem?

Thanks.