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Downloading FASTQ with aspera

Hello everyone

I used to download FASTQ files form ENA server with aspera, but I cant download datas anymore.

This code is what I used to use. (Thanks to SRA explorer)

ascp -QT -l 300m -P33001 -i $HOME/.aspera/connect/etc/asperaweb_id_dsa.openssh era-fasp@fasp.sra.ebi.ac.uk:vol1/fastq/SRR581/008/SRR5817038/SRR5817038_2.fastq.gz . && mv SRR5817038_2.fastq.gz SRR5817038_GSM2699772_8858-3_Hepa_Day_130_bulk_Homo_sapiens_RNA-Seq_2.fastq.gz

after some times, there was no download start signal nor error log. just new command line starts.

I tried to eliminate -P33001 parameter then it shows

ascp: Failed to open TCP connection for SSH, exiting.

Session Stop  (Error: Failed to open TCP connection for SSH)

I allowed port 22 and port 33001 with ufw long time ago.

I changed my internet to figure out whats going on, but it doesn`t work.

Is there any download policy change from ENA server?

Thank you very much.

sra

It is possible that aspera is down as someone else was having trouble with it yesterday. Try plain http/ftp links for now.

Tyvm.

Seems like ENA sever issue.

Yes. Few days ago ENA posted about they going to upgrade and it may affect data download. It may take 7-10 days to fully restore.

1 answer

Currently now not (sorry for the typo) possible, see Ensembl reduced functionality: 10th March-4th April 2020

looks like ascp sever is still down. You can find the comment about it too. Are you able to download via ascp? Thank you

@ATPoint should have said NOT possible.

ascp now working for me as of Apr 9th!

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