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T-test analysis for gene expression comparison

Hi there, I would like to perform a t-test to evaluate the gene expression level between the mutation and normal groups. Can anyone prefer a suitable package and guidelines?

rna-seq
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updated by bioinformatics2020 bioinformatics2020 • written by Nithya • Nithya

What type of analysis is this?

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written by rpolicastro rpolicastro

A statistical student's t-test to compare gene expression levels between normal and mutation tissue samples.

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written by Nithya • Nithya

1 answer

You can't publish with a t-test result, it's just not a smart enough way to handle the math involved.

Do what everyone does. Run DESeq2 or EdgeR in R.

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written by swbarnes2 swbarnes2

THIS! You need to go back and learn about what type of distribution is used for a t-test. Then start understanding the distributions that gene expression data follows. This will give you a quick insight as to why you cannot use a t-test, and why the tools swbarnes2 posted about are needed.

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written by bioinformatics2020 bioinformatics2020

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