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A question on gene expression analysis

Dear all,

I assume that I have 2 groups: Cancer and Normal samples. Are there any differences when I perform gene expression for Cancer vs Normal and Normal vs Cancer? I would like to use random effects model and compute the combined effect size. If possible, please clarify the differences.

Thank you in advance.

microarray gene expression

1 answer

"Cancer vs Normal" and "Normal vs. Cancer" will have the same results, with the latter having fold-changes with opposite signs of how people normally think about things.

Dear Ryan,

You mean it is just about 5 or -5 when we have the opposite comparisons (A to B or B to A), right?

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