How To Add Short Sequence To The Beginning/End Of All Reads?
Hi there!
I have fastq files and I would like to add short sequence at the beginning/end of all reads. There are plenty of ways how to trim sequences, but I have not found anything for appending :-) All added nucleotides can have arbitrary quality score.
Any ideas? Thank you.
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Biomonika (Noolean)
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Do you just want to add some text? If so, you could just use sed, awk, perl, or any other language really..
Yes, I have just written script that does such think in perl :-)
perhaps post that as an answer and see what people think. Trimming is a much better defined problem it is clear why people want to trim. Adding to a sequence is both unusual and a very domain specific need as such I know of no tool that offers that directly.
Then why are you asking this question? For other implementations? And why would you want to do this?