Dear All,
Do you know any tool to predict supersecondary structure in proteins?
thank in advance.
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Jamand
You can use Threader and its derivates. It can statistically recognize the fold of your protein/domain. It's pretty slow, but quite reliable.
fold != supersecondary structure. To the best of my knowledge THREADER is a fold recognition program, not a supersecondary structure prediction program.
That's true. Nevertheless, you can use it for SSS prediction if you don't have a target in mind (e. g. transmembrane helices). You just need to divide your sequence in appropriate pieces. It's quite brute force, it's slow but works. Test it yourself using some known pi-helices. Besides this approach, I don't know a generic SSS server/program.
Not sure if there is a consensus prediction program which can predict various supersecondary structures (for example Helix-Turn-Helix, Beta-hairpins etc.) from sequence or structure information. Structure prediction is an area of active area research and various algorithms are available. I would recommend you to make a list of your supersecondary structures of interest and then search for servers/program which could predict such features from sequence information.
For example:
Helix-Turn-Helix prediction: http://npsa-pbil.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSA/npsa_hth.html
Beta-hair pin prediction: http://www.imtech.res.in/raghava/bhairpred/supli.html
Also refer to Chou K. C's publications and various secondary structure prediction servers from G. P. S. Raghava's group for a good background reading on available supersecondary structure prediction algorithms.
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Khader Shameer
Khader, your links proved much more useful than I thought initially! I've caught myself using Raghava's servers regularly by now (and recommending).
Try out the following tools
These are the best tools
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Anuraj Nayarisseri •
Anuraj: MotifScan and iprscan is for functional motif prediction. jamAnd is looking for supersecondary structure information, not functional motifs/patterns.
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What is your data type ? Sequence or Structure ? Are you looking at any specific type of supersecondary structure ?