Hi,
I am currently looking into four proteins that are supposed to interact with each other. I would like to find out what exact residues are responsible for the interaction, the only problem is that I don't have a structure for any of them. All of the algorithms I've found require a known structure. I was wondering if there is a way to do this without structure (i.e. based on alignments) or if there is a way to predict the 3D structure to then model their interactions?
Note that results do not need to be perfect, I would just like to infer likely residues responsible for the interactions so that I can then mutate the proteins in downstream wet-lab experiments and confirm whether these residues do in fact mediate the interactions.
Thank you in advance for any help.
Hey michael, sorry for the late response. Have you looked at any of the tools listed at the Protein Model Portal? In particular, look through the Protein Modeling 101.