Hi,

I am currently looking into four proteins that are supposed to interact with each other. I would like to find out what exact residues are responsible for the interaction, the only problem is that I don't have a structure for any of them. All of the algorithms I've found require a known structure. I was wondering if there is a way to do this without structure (i.e. based on alignments) or if there is a way to predict the 3D structure to then model their interactions?

Note that results do not need to be perfect, I would just like to infer likely residues responsible for the interactions so that I can then mutate the proteins in downstream wet-lab experiments and confirm whether these residues do in fact mediate the interactions.

Thank you in advance for any help.