I've generated a wig file for the chr22. The data is a sliding window (window-size=500, every 250pb) starting from pos0=16050276. The windows overlap over 250pb.

$ head input.wig
fixedStep chrom=chr22 start=16050276 step=250 span=500
4
3
5
5
2
3
3
2
3

there is only one header in my file:

$ grep -i -c Step input.wig 
1

and there is 140777 lines (including the header)

$ wc -l input.wig 
140777 input.wig

length(chr22)=51304566

$ grep chr22 sizes.tsv 
chr22    51304566

but when I want to convert my wig to a bigwig file using wigToBigWig, I get the following error:

wigToBigWig input.wig sizes.tsv out.bw
There's more than one value for chr22 base 16306276 (in coordinates that start with 1).

I must be very tired, do you know where is my error ?