hisat2-build multiple fasta and Annotation
I am using 3 extra sequence with GRCh38 as my sample have some vector with gene in it for indexing I did
hisat2-build -p 16 seq1.fa, seq2.fa,GRCh38.fa Genome
my question is if I insert new sequence in reference and align, then how I will annotate the same when I am using standard GTF file?
How Can I update my GTF file or what is the approach for annotation?
• 1,009 views
•
link
written
by
chimerajit •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Help - how to merge human reference genome and GTF file with a custom sequence.
written by Dyno •
-
How to construct a phylogenetic tree accroding to sequence similarity matrix
written by Jason •
-
Multiple Sequence Alignment using HMM
written by anasjamshed
-
HISAT2 Error Building Index
written by adam.faranda
-
How to build genome index file with ERCC (spike-in )
written by Mike
-
How to bring consistency for genome reference, annotation and index files?
written by Vasu
-
RNA-seq mapping Reference genome
written by k.kathirvel93
-
Primary assembly or patches: what to choose for making index file?
written by piyushjo
-
building index with HISAT2
written by blooming.daisy333 •
-
Minimal CWL (Common Workflow Language) workflow from Makefile
written by Pierre Lindenbaum
