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To construct a phylogenetic tree using Biopython, you can use the DistanceTreeConstructor class from the Bio.Phylo.TreeConstruction module. This class provides methods to construct a phylogenetic tree using distance matrix. Here is a simple example:

from Bio.Phylo.TreeConstruction import DistanceTreeConstructor
from Bio.Phylo.TreeConstruction import DistanceMatrix

# Your sequence similarity matrix
matrix = [
    [1, 0.9, 0.6, 0.4],
    [0.9, 1, 0.5, 0.1],
    [0.6, 0.5, 1, 0.6],
    [0.4, 0.1, 0.6, 1]
]

# Names of your sequences
names = ['Seq1', 'Seq2', 'Seq3', 'Seq4']

# Create a distance matrix
dm = DistanceMatrix(names, matrix)

# Create a DistanceTreeConstructor object
constructor = DistanceTreeConstructor()

# Construct a tree using UPGMA algorithm
tree = constructor.upgma(dm)

# Print the tree
print(tree)

In this example, the upgma method is used to construct the tree using UPGMA (Unweighted Pair Group Method with Arithmetic Mean) algorithm. You can also use the nj method to construct the tree using NJ (Neighbor Joining) algorithm.

Please note that the distance matrix should represent the evolutionary distance between the sequences, not the sequence similarity. You might need to convert your sequence similarity matrix to a distance matrix before constructing the tree.