Thanks, I agree it works fast. Shame it doesn't take a file with a whole list of regions. But I can put it into a loop.
Hello all,
Sorry if this has been answered before (though I have not found an appropriate answer already) What is an effective way to extract mutliple chromosomal regions from a vcf.gz file? I see that it can be done for single regions using tabix, but I have a file with several regions in a text file in the format:
chromosome:startposition-endposition eg: 19:47366525-47380539
Perhaps there is a way to refer to a file of regions using tabix, or vcf-tools? If anyone could provide an example command line that would be really helpful.
Thanks in advance!
8 answers
I find tabix to be much easier / much sorter command line arguments.
You don't need a fasta for this method. Tabix is screaming fast.
bgzip your.vcf
tabix -p vcf your.vcf
tabix your.vcf.gz chr1:10,000,000-20,000,000
Two ways: 1) if there are many regions: tabix -fB my.vcf.gz reg.bed 2) if you only have a handful of regions: awk '{print $1":"($2+1)"-"$3}' reg.bed | xargs tabix my.vcf.gz.
For anyone arriving years later, the command appears to have changed. The command you probably want is: tabix -R reg.bed my.vcf.gz.
None of these work anymore.
tabix -R region.txt my.vcf.gz
worked for me
Would anyone be able to let me know what the input is for the region.txt file? I have put 57646425-57803821 (all in one line). Linux is telling me could not parse bed line. Thank you for your help.
Have you tried to add the chromosome position in the beginning? eg: chr1:57646425-57803821
Another way is separate by tab. eg: chr1 57646425 57803821
For others if you like to use BED files, one way to do this would be like so:
vcftools --vcf input.vcf --bed bed_file_describing_the_range.bed --out output_prefix --recode --keep-INFO-all
Where bed_file_describing_the_range.bed has the positions of interest.
I am trying to extract specific regions from a VCF file that has SNP information for 16 individuals. My bed file has three columns (scaffold name, start of region, end of region).
When I run vcftools on these files, it runs through the entire vcf file and determines that there are no regions from the bed file contained in the vcf file. A recode vcf file is output, but it only contains the header information from the vcf file.
Check to ensure your scaffold names match in the BED and VCF files. For example, if your BED file has "chr1" but your VCF file just has "1" for chromosome 1 it will return an empty file.
bedtools intersect worked better to me
intersectBed -a input.vcf -b /path/to/my.interval.bed -header > output.vcf
For some reason by intersected out VCF have more line compare to input vcf. :( :( No idea what could be the possible reason for this !! Any guess ?
Another straightforward option would be with bcftools: https://samtools.github.io/bcftools/bcftools.html#view
As a general example like this: bcftools view -R yourRegionFile -o outFile yourVCFfile
Interestingly the region file "yourRegionFile" can be specified either on command line or in a VCF, BED, or tab-delimited file (the default). See the bcftools manual for regions file here: https://samtools.github.io/bcftools/bcftools.html#common_options
Nowadays I use this option
docker run -v $PWD:$PWD -w $PWD biocontainers/bcftools:v1.9-1-deb_cv1 bcftools view-R yourRegionFile -o outFile yourVCFfile
bcftools view -R regions.bed your.vcf > output.regions.vcf
You could use the GATK SelectVariants Walker, specifying the -L option to set the intervals your are interested in.
Here is an example of how to do that, taken from the link above:
#Select a sample and restrict the output vcf to a set of intervals:
java -Xmx2g -jar GenomeAnalysisTK.jar \
-R ref.fasta \
-T SelectVariants \
--variant input.vcf \
-o output.vcf \
-L /path/to/my.interval_list \
-sn SAMPLE_1_ACTG
Note that you have to have a fasta reference file with the same names and coordinates as you use in our region file and vcf, and that you can remove the -sn option if you want to extract the variations for all samples.
Hope this helps.
thanks, I can potentially use this, but having some issues with the reference fasta, so a method that does not require a fasta reference would be preferred.
I'm not sure if it requires uncompressing the vcf files. I'm not that familiar with vcftools, so I couldn't say if there is an easier way to do it using that suite.
bedtools intersect worked better to me
intersectBed -a input.vcf -b /path/to/my.interval.bed -header > output.vcf
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Use
tabix my.vcf.gz chromosome_name:start_position-end_position -h > out.vcf