Hi,

I did KEGG pathway GSEA of 10 different gene expression datasets (5 of disease 1 and 5 of disease 2).

Now, I would like to know if there are pathways enriched in opposite directions in disease 1 versus disease 2 (for example up-regulated across datasets of disease 1 and down-regulated across datasets of disease 2).

Is there any method to calculate which pathways are significantly enriched across multiple datasets using GSEA summary statistics?

Many thanks