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Can anyone suggest me the recent and best pipeline for RNA-Seq data analysis

I am working on grapevine whose genome is recently available. So, I will be mapping my reads to genome. (It is reference based).

I will be mainly checking the differential gene expression analysis at different different time points.

which is the most advanced pipeline these days? can anyone suggest me

rna-seq

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PS: effectively a duplicate of Can anyone suggest me the recent and best pipeline for RNA-Seq data analysis

Hi Hershraje

For DEG you can use Hisat2 or STAR for Alignment,feature-count or HTseq-Count for extracting genes count table,and EdgeR for differential expression analysis.

good luck.

Lmira

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