Can anyone suggest me the recent and best pipeline for RNA-Seq data analysis
I am working on grapevine whose genome is recently available. So, I will be mapping my reads to genome. (It is reference based).
I will be mainly checking the differential gene expression analysis at different different time points.
which is the most advanced pipeline these days? can anyone suggest me
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Hello harshraje19!
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Hi Hershraje
For DEG you can use Hisat2 or STAR for Alignment,feature-count or HTseq-Count for extracting genes count table,and EdgeR for differential expression analysis.
good luck.
Lmira