Speed vac for RNA-Seq
I was wondering if concentrating the RNA by using a speed-vac on a low-temperature setting would have any effect on the quality of the DGE. Does anyone here have experience with this?
• 533 views
•
link
written
by
jahnic.beck •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Bulk RNA seq analysis on multiple conditions (4)
written by SO-YEON •
-
Classification for low quality variant on IGV
written by hello_bioinf •
-
Heirarchal regression for model comparison, RNA-seq
written by RNAseqer
-
Deletions together with SNVs on BAM files
written by baliczap •
-
Extracting Unmapped Reads from BAM files using Rsamtools
written by dk0319 •
-
Absolute Copy Number from low pass WGS
written by senowinski •
-
Differential Peak Expression in ATAC-seq data
written by ccag •
-
Creating a tree from pairwise FST values for ADMIXTURE components
written by devenvyas
-
Low input / single cell RNA-seq with kits
written by Genosa
-
Accurately Computing Melting Temperature Of 10-Mer Rna-Rna Hybridization
written by ericmajinglong
Hello jahnic.beck!
We believe that this post does not fit the main topic of this site.
Please ask this at StackExchange Biology, SeqAnswers or a suitable subReddit as it is not bioinformatics-related.
For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.
If you disagree please tell us why in a reply below, we'll be happy to talk about it.
Cheers!
Besides that...I would never put my RNA into any machine that requires no lids on the tubes. Better put it over a purification micro column or concentrate with RNA-binding beads. Anything is imho better than speedvac-ing.
As far as i know, If the RNA Integrity Number (RIN) does not drop below 7.0, you should not have any problem.