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normalization of Agilent data

Hello evry body, We couldn’t input the Agilent data into the flex array. So we used R software for normalize the data, and then plot it in flex array but it didn’t work as well. Now I am wondering if we should use the same package for plotting as we used for data normalization!? we would be grateful if you help us

r agilent flex array
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updated by Biostar Biostar • written by sh • sh

What is the Agilent data, what is R software (=which package are your referring to), how did you normalize and what did not work? Please read the limma documentation which is commonly used for array data (assuming it is gene expression array), apply the code there and then come back with specific questions and the necessary details.

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written by ATpoint ATpoint

my data is micro array data, i used the limma package to normalize the data and run the struction of boxplot that shows data is normal then i inserted the R output into flex array. there showed the input data is not normal.

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written by sh • sh

Add the code you are using, this may help us to find the possible error.

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written by Leite Leite

Hello sh.o.94!

We believe that this post does not fit the main topic of this site.

Not enough details.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

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written by ATpoint ATpoint

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