Hi every one

I doing a friend a favor about his project,

He has only 2 dataset, one of them is control and one is treat, those are pair end data and he does not had the budget to send more sample for rna sequencing. So anyway i did align those data and finally i cant find any tool for differential expression, i tried limma edgeR and deseq2, even salmon i tried out.

Is it even possible ? and if it is, suggest me which tool.

P.S i worked with Star and then featurecount

Thanks