Hi every one.
i did rna seq analysis. in PER3 gene and PER4 pseudogene i didnt get any counts, but in IGV i saw some small peaks which means they have some counts, even very low, but still they have !!. i want to know is there any way i can get any counts from that ? were my options in my aligner wrong or what else ?
i used blood samples. and used with trimmomatic, STAR, featureCounts