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How to add short sequences to a database using makeblastdb

How can I get makeblastdb create a database with short sequences from my .fasta file?

sequencing alignment sequence

Does this work?

makeblastdb -dbtype nucl -title "Short sequences" -in sequences.fasta -input_type fasta -out DBshort

The process shouldn’t differ from a normal blastdb creation. Have you encountered an error or a problem you’ve not shown us?

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