umi_tools counts option
Hi, I have worked used umi_tools and It's interesting to see deduplication results. In the single cell tutorial a method called counts has been mentioned, My question is can I do counts for miRNA seq data or is it only single cell-specific? I tried counts with option per gene but it has not retrieved any results. what I am looking for is a list of miRNAs with umi number and read counts.
Thanks, Hothri
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Does your experimental setup incorporate UMI's?
yes, we are using QIAseq kit, they do this kind of analysis with their software called gene globe. I am trying to do the similar with umi_tools