comparing STARsolo UMI-based directional deduplication vs umi-tools?
According to the STARsolo docs, setting --soloUMIdedup 1MM_Directional_UMItools:
follows the "directional" method from the UMI-tools
Can anyone confirm how this differs from calling umi-tools dedup like:
# Note that by default, `--method=directional` for umi-tools.
umi_tools dedup --per-cell --extract-umi-method=tag --umi-tag=UR --cell-tag=CR \
-I $input_bam \
-S $output_bam;
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I haven't done extensive benchmarking, but the claim from the STARsolo authors is that it should be the same.
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