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I am trying to visualize my Bigwig file with UCSC or IGV, about UCSC I don't know any about constructing custom tracks and can't do it, and IGV can't show it. how do I Construct a custom track in UCSC? or about IGV?
Have you loaded the correct genome in the IGV? This tutorial might help.
thanks for your reply.
yes, I have. I think IGV cant open BigWig file, it works just with convert it's format to TDF.
I can confirm that IGV can open BigWig files, I have used it in past with BigWig files, are you sure that your files are in correct format?
IGV definitely supports BW format. Please check if you have the current version of IGV, and point out how these BWs have been generated, and what the error message is.
FYI I was having trouble also until I made sure the extension was .bw from call to bedGraphToBigWig
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