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Is it correct to merge bigwig with TPM values from rnaSeq from different biological replicates for the same condition ?

STAR can give output in bigwig for each strand with RPM values for each biological replicate.

Is it correct to merge them to visualize them in one track ? or does it corrupt initialy computed TPM values ?

is it better to look separately to each replicate or should I just merge raw counts ?

I'd like to have only one track for each condition in the viewer (IGV, or UCSC...) and not several tracks per condition.

rna-seq wig bigwg visualisation
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updated by Satyajeet Khare Satyajeet Khare • written by ZheFrench ZheFrench

1 answer

I would keep biological replicates separate and use them to test my hypothesis. You can use any one of three replicates to generate a figure. If the event you want to display on one track, is statistically accurate, it should be present in individual replicates too.

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written by Satyajeet Khare Satyajeet Khare

Is merging the str1 and str2 bigwig files with RPM correct?

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written by lcscs12345 • lcscs12345

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