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DNA size on Miseq

Would you please guide me about the size dependency of illumina Miseq ? Is it possible to sequence ~6MB DNA using Miseq?

genome next-gen sequencing
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updated by ATpoint ATpoint • written by ambrinaakbar • ambrinaakbar

Yes, easily - but its not quite that simple.

You can get roughly 30 bacterial genomes (about 4.5-5 Mbp), at 30X coverage from a MiSeq run. If you want higher coverage, it obviously drops.

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written by Joe Joe

Hello ambrinaakbar!

We believe that this post does not fit the main topic of this site.

This question does not primarily relate to bioinformatics and would probably more appropriate on http://seqanswers.com/

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

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written by WouterDeCoster WouterDeCoster

1 answer

Yes, but it depends on the desired coverage. Use the Illumina Coverage Calculator to help planning your experiment. E.g. given your 6Mb genome, with a desired coverage of 50x and 5% expected duplicates, you could pool ~23 samples with one v3 2x150bp kit.

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written by ATpoint ATpoint

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