Off topic:Fragment size for Illumina Miseq 2 x 250-bp sequencing

Question

[kat]

I am simulating Illumina MiSeq 2 x 250-bp data with ART (https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm) and am wondering the optimal fragment size (mean and st. dev.) for sequencing on this platform. Is there a recommended fragment size or insert size for shearing DNA prior to sequencing? Thank you!