Fragment size for Illumina Miseq 2 x 250-bp sequencing
I am simulating Illumina MiSeq 2 x 250-bp data with ART (https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm) and am wondering the optimal fragment size (mean and st. dev.) for sequencing on this platform. Is there a recommended fragment size or insert size for shearing DNA prior to sequencing? Thank you!
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Hello kwalter!
We believe that this post does not fit the main topic of this site.
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