Stand alone balst+
I am doing stand alone blast i want set query coverage, how to get the query best hists above 60%
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You may filter after blasting, if you use genomax suggestion, or you may use the -qcov_hsp_perc parameter (you may also want to use -max_hsps):
-qcov_hsp_perc <real, 0..100="">
Percent query coverage per hsp
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Have you looked at the format options for NCBI blast. Especially
qcovoption inoutfmt.my query file vary with sequence size result all so showing less coverage to that sequence i am no getting proffer result to sequence simlarity