Blastp for virulence factors
Hello I am trying to use my RAST annotated amino acid file to identify virulence factors in my E. coli isolates. I am performing stand alone blastp against VFDB database of E. coli. Can you pls suggest me what is a desirable pident cutoff to be set after extracting the best hits. Thanks in advance.
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E-value would probably be a better paramter to identify the VFs.