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DE gene analysis

I performed differential gene analysis on two GEO datasets using NetworkAnalyst , i found set of up- and down- regulated genes using Fisher's method. Since I am new to the area of analysis , i wanted to know how to further proceed with the analysis to find a biomarker Thank You!

de genes

1 answer

Gene set enrichment analysis is a good next step. You can do a contrast based analysis with something like EGSEA, or you can do per sample enrichment scores with GSVA.

Hii! Is there any codes available for doing the enrichment analysis using EGSEA in R?

Yes. The package has plenty of documentation and working code samples.

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