Multiple Sequence Alignement

Question

[pradeep13march]

Hello i want to know that generally for find protein conserve domain by the multiple sequence alignment but for find conserve domain if first i will convert these protein into m-rna and then MSA of among these m-rna sequence. after MSA the mrna region which will we be conserve in result, can be use these conserve mrna sequence for modelling a protein.

Thank you

[Michael]

Hi, it is not clear what you are asking. Please take your time to reformulate your question using standard english.

[pradeep13march]

sir i want to know that can i use mrna sequence of any proteins for find out conserve domain. (Multiple Sequence alignment ).

[Benn]

Conserved regions are more likely to be found in proteins. Because of synonymous variations (mutations) in DNA/RNA you can better not use the DNA/RNA sequences but first translate it into protein sequences.

[GenoMax]

if first i will convert these protein into m-rna and then MSA of among these m-rna sequence

pradeep13march : If you already have protein sequence why are you back translating that into mRNA?

Take a look at this guide before reformulating your question: How To Ask Good Questions On Technical And Scientific Forums