Thanks for your answer. Can I know the meaning of * in the MSA? I think it is different from gap '-'?
Here is one example when I downloaded the gene tree for ENSG00000211626, there is a * in this protein.
>Mus caroli strain CAROLI_EIJ, Ryukyu mouse, MGP_CAROLIEiJ_G0000075, MGP_CAROLIEiJ_P0000104
-MVFTPHIL----------------GLLLF*ISASTGDILMTQSPATLSVTPGETVSLSCRASQSINKNLHWYQQKSHGSPRLFIKYASDSISGIPSRFTGSGSGTDYTLSINSVKPEDEGIYYCHQCYSTPNN-
As a follow-up question, can we download a sub-tree including fewer species? Thanks!
Yes you can.