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How to identify the different varieties of same species in Plants?

Dear All I want to establish a system to differentiate between the varieties of same species(specifically plants). Please let me know if anyone has done it with any protocol.

Best Regards Shahzad

genome sequencing sequence next-gen

Sounds like a very good name for a phd project :D It depends on what data you have!

@Macspider Thank you for the reply. I am starting this project. So, I can opt any method. I have experience with Next Gen data and also have molecular biology skills. I just want to enlist all the options which can lead to success of this project.

1 answer

Since this is exactly my project as well (lol) I would suggest you to first have a look to:

  • differential expression if you have RNASeq data
  • variants if you have DNA Seq data
  • chromosomal rearrangements if you have assemblies available
  • GC content and contamination content

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