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How to sequence a gene along with its 3' and 5' UTR

Dear All I have a gene transferred into a plant (cotton) chromosome. I want to know the upstream and downstream DNA sequences present with this gene. Please guide me the possible procedure/s by which I can get this information and specially is there any possibility that NGS can help in this matter. If anymore information is needed please ask.

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gene sequence utrs
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updated by Carlo Yague Carlo Yague • written by Shahzad • Shahzad

Do you want the flanking DNA regions (not part of the transcript) or the UTRs which are part of the gene and are transcribed ?

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written by Carlo Yague Carlo Yague

I want the sequences of flanking DNA regions(upstream and downstream) to know the place of this Gene in a chromosome.

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written by Shahzad • Shahzad

If you know the sequence of your inserted gene, you could try "cycle sequencing" by designing a single primer at the end of your gene that will read into the flank (and then do the same for the start).

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written by mbyvcm mbyvcm

Thank you all for these wise suggestions.

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written by Shahzad • Shahzad

1 answer

NGS can definitely help if you do genome sequencing. This thread may give you some ideas on how to proceed with the data.

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written by Carlo Yague Carlo Yague

please also let me know if there is Any other technique which can earmark the transformed gene's position in chromosome so I can get the up and downstream DNA sequences?

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written by Shahzad • Shahzad

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